Search found 2 matches

by pedrochang
Tue May 20, 2008 1:30 am
Forum: Molecular Biology
Topic: 6X His Tag vector construction assistance needed
Replies: 2
Views: 8097

Re: 6X His Tag vector construction assistance needed

hi snowcapk

Sorry for the delay. Thanks very much for your explanation. Was hyper-paranoid and decided to use quickchange to insert the 6X His between gene of interest and the signal sequence. Hope it remains 6X. Thanks again.

pedro
by pedrochang
Fri May 02, 2008 12:24 am
Forum: Molecular Biology
Topic: 6X His Tag vector construction assistance needed
Replies: 2
Views: 8097

6X His Tag vector construction assistance needed

Hi Everybody, New to the forum. First time post, so greetings. I am designing a vector that starts like this : ( backbone - ATG -Signal sequence - MCS - gene - MCS - TGA - backbone ) and i want to make ( backbone - ATG -Signal sequence - 6xHis - MCS - gene - MCS - TGA - backbone ) my question to all...