Search found 2 matches

by koze99
Wed Apr 23, 2008 1:52 pm
Forum: Molecular Biology
Topic: Comprative Quant PCR method on RNA question
Replies: 0
Views: 879

Comprative Quant PCR method on RNA question

I understand that 99% of the RNA isolated with my total RNA isolation kit (qbio problu kit) is rRNA. I am going to quantitate mRNA for specific genes and compare to 16S expression. Do my 16S primers amplify only mRNA for 16S or also all the rRNA? Stupid question maybe but I am confused on some recen...
by koze99
Thu Mar 27, 2008 1:51 am
Forum: Molecular Biology
Topic: Heterologous expression of Archaeal genes,
Replies: 0
Views: 1332

Heterologous expression of Archaeal genes,

Hi, I am interested in the Heterologous expression of an Archaeal gene from a sulfolobus/metallosphaera like species. The organism is a thermo acidophile. Has anyone had any luck using a vendor for archaea or can someone recommend a service. Promab is one company I am considering. They charge 1800 d...