Search found 7 matches

by shivakumar
Tue Apr 28, 2009 5:52 am
Forum: Molecular Biology
Topic: constuction of a full length gene
Replies: 1
Views: 1593

constuction of a full length gene

hi everybody
how to construct a full length gene from partial length cDNAs available from sutracted cDNA libraries. What are the techniques involved in it. And suggest me some related websites, plz.

thanks a lot in advance
by shivakumar
Mon Dec 22, 2008 5:44 am
Forum: Molecular Biology
Topic: unit conversion
Replies: 1
Views: 1283

unit conversion

how can I convert picomoles to nanograms?
by shivakumar
Thu Dec 18, 2008 10:40 am
Forum: Molecular Biology
Topic: Colony PCR
Replies: 2
Views: 2899

Colony PCR

I did a colony pcr reaction (final vol 100ul) and the product was electrophoresed in 1.2% agarose gel . After complete run I observed a smear from one end to another end in each well (of the gel) including blank (without template). I thought that problem with concentration of DNA template and tried ...
by shivakumar
Wed May 14, 2008 11:18 am
Forum: Molecular Biology
Topic: PCR
Replies: 1
Views: 1312

PCR

hi everybody In order to study the role of some genes, I have designed gene specific primers and made a PCR. And I separated the amplified products in agarose gel and got bands. Surprisingly I got the same bands with the PCR product which was done without using cDNA template(which was for the confir...
by shivakumar
Wed Apr 16, 2008 4:44 pm
Forum: Molecular Biology
Topic: role of stress related genes
Replies: 2
Views: 1927

role of stress related genes

can anybody tell the role of following stress related genes like
ERD4, ACT domain, Ascorbate peroxidase and zink finger in plants
by shivakumar
Wed Apr 02, 2008 4:28 am
Forum: Molecular Biology
Topic: m RNA purification
Replies: 1
Views: 1171

m RNA purification

hello everybody
In a protocol of mRNA purification from RNA(Horsegrm plant)
I found magnetic beads in the finnal centrifugation step as a pellet(along with RNA pellet). How to remove those
particles.
by shivakumar
Fri Mar 14, 2008 6:29 am
Forum: Molecular Biology
Topic: Can any one clarify my question with DNA
Replies: 2
Views: 4006

Can any one clarify my question with DNA

hi everybody

while extracting DNA, extraction buffer containing
NaCl, EDTA, Tris n CTAB
whts d role of those chemicals n
n why adjusting d buffer pH to 8

n I feel same doubt with chloroform isoamyl alcohol(their role)