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by Jfangy8
Tue Aug 20, 2013 12:53 am
Forum: Molecular Biology
Topic: hydroxyproline assay to determine collagen content
Replies: 5
Views: 13308

Hello ucsfmed2012,
How do you treat your samples(100ul of supernatant from each sample)? Do you add 100 μl
concentrated HCl (~12N, not provided) in a pressure-tight, teflon capped vial and hydrolyze at
120°C for 3 hours?

Thanks!
L.