Search found 2 matches

by reulita10
Tue Jul 16, 2013 12:35 pm
Forum: Molecular Biology
Topic: calcium measurement- help!!
Replies: 0
Views: 1430

calcium measurement- help!!

Hello everyone, i'm looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght). I understand the equation with Kd, Fmin, Fmax etc. but don't know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA ...
by reulita10
Tue Jul 16, 2013 12:34 pm
Forum: General Discussion
Topic: calcium measurement- help!!
Replies: 0
Views: 1213

calcium measurement- help!!

Hello everyone, i'm looking for ideas and protocols how to measure Ca+2 conc. by using the fluorofore Flue-3 (single exitation wavelenght). I understand the equation with Kd, Fmin, Fmax etc. but don't know exactly how to do it: what conc. should I use for Ionomycin? and EGTA? and when does the EGTA ...