Search found 9 matches

by unibas
Sun Mar 06, 2011 12:35 pm
Forum: Molecular Biology
Topic: PCR question
Replies: 3
Views: 2371

thanks! I'll do that. follow up question: suppose the primers remain bound after one hour at room temperature. after this one hour, is it possible to release the primers from the plasmid? I guess if I just heat it to 95 and cool it again, the primers will get back on the plasmid. but I don't want th...
by unibas
Sun Mar 06, 2011 10:35 am
Forum: Molecular Biology
Topic: PCR question
Replies: 3
Views: 2371

PCR question

what happens if after the first denaturation and annealing steps, I take the tube out of the PCR machine and leave it at room temperature for an hour or so? would the primers remain stuck to the template? if not, how can I make sure the primers remain on the template for about an hour? this is not a...
by unibas
Tue Oct 19, 2010 11:05 am
Forum: Molecular Biology
Topic: What temperature is needed to destroy RNA?
Replies: 4
Views: 3188

ok good, I'll try that. thanks!
by unibas
Tue Oct 19, 2010 10:29 am
Forum: Molecular Biology
Topic: What temperature is needed to destroy RNA?
Replies: 4
Views: 3188

yes, I know.
but I can temporarily increase the temperature in a very limited space within a cell, and I want to know what temperature I need to reach in order to destroy mRNA.
by unibas
Tue Oct 19, 2010 9:05 am
Forum: Molecular Biology
Topic: What temperature is needed to destroy RNA?
Replies: 4
Views: 3188

What temperature is needed to destroy RNA?

Hi, does anyone know by any chance what temperature is needed in vivo to destroy mRNA? thanks.
by unibas
Wed Nov 28, 2007 9:27 pm
Forum: Molecular Biology
Topic: glass vs. plastic when working with lipids
Replies: 3
Views: 2223

I suppose lipids stick to plastic. I think most of the protocols for lipid analysis ask for glass tubes.
by unibas
Wed Nov 28, 2007 8:59 pm
Forum: Molecular Biology
Topic: glass vs. plastic when working with lipids
Replies: 3
Views: 2223

glass vs. plastic when working with lipids

Hi, I need to analyze the fat content of stool samples from mice. I found a protocol that involves chloroform:methanol extraction and weighing the extracted fat. The entire protocol is with glass tubes, but we don't have a centrifuge that fits glass tubes. How important is it to use glass and not pl...
by unibas
Tue Aug 15, 2006 12:30 pm
Forum: General Discussion
Topic: mouse ear clipper?
Replies: 0
Views: 2147

mouse ear clipper?

Hello, We are new to working with mice. We tag our mice by ear clipping, and use the small piece of ear for genotyping. We have tried several clippers, but they are not ideal since they never puncture a full hole, there is always a small piece which stays attached. Then we have to play with the angl...
by unibas
Tue Aug 09, 2005 8:06 am
Forum: Cell Biology
Topic: non-radioactive ATP analog for kinase assay?
Replies: 1
Views: 2513

non-radioactive ATP analog for kinase assay?

Hello I am trying to check wether a certain protein is a substrate for the mTOR kinase in mammalian tissue culture (NIH 3T3 cells). I want to feed the cells with labeled ATP and then IP the protein and see if mTOR inhibition would change its phosphorylation status. I really don't want to use such a ...