Search found 35 matches

by ss_kalve
Wed Mar 18, 2009 5:42 am
Forum: Molecular Biology
Topic: Protein extraction from leaf tissue
Replies: 0
Views: 1514

Protein extraction from leaf tissue

Dear all,
can anybody tell me the procedure to extract protein from plant leaf tissue by sonication.actually i just want the buffer composition & frequency,temperature and duration of sonication.Further i want to proceed with 2D electrophoresis with this isolated protein.
by ss_kalve
Tue Feb 24, 2009 1:04 pm
Forum: Molecular Biology
Topic: extraction buffer for native page
Replies: 0
Views: 1011

extraction buffer for native page

do anybody know the extraction buffer composition for the protein by which native can be done in plant samples.is this different from the sds page or the same?
by ss_kalve
Mon Feb 16, 2009 7:49 am
Forum: Molecular Biology
Topic: Operon info
Replies: 4
Views: 2977

and one important thing saccharomyces cerevisiae is also an eukaryotes.so it shoul have similarity with fern.
by ss_kalve
Mon Feb 16, 2009 7:31 am
Forum: Molecular Biology
Topic: Operon info
Replies: 4
Views: 2977

Re: Operon info

Dear, you are correct that prokaryotes and eukaryotes having different mechanism but as far reported saccharomyces cerevisiae having similarity with plant systen and this Ars operon is reported in saccharomyces cerevisiae also. so there are chances of getting this operon in fern i.e. plant system. s...
by ss_kalve
Sat Feb 14, 2009 1:58 pm
Forum: Molecular Biology
Topic: Operon info
Replies: 4
Views: 2977

Operon info

Dear,
i've one operon information which is present in one system i.e. Ecoli and Sachharomyces and i would like to know if this operon is present in another system i.e. a fern or not,is this possible?bcoz i dont know the sequence of fern, its not reported till now.
by ss_kalve
Tue Jan 27, 2009 9:42 am
Forum: Molecular Biology
Topic: native page
Replies: 0
Views: 1089

native page

Does anybody do native page?plz give me the details or any protocol you use in ur lab.
by ss_kalve
Tue Jan 20, 2009 4:29 pm
Forum: Molecular Biology
Topic: Protein degradation
Replies: 5
Views: 5354

Re: Protein degradation

Dear, thnx for reply.actually as i tld i've precipitated protein by acetone precipitation. In this precipitation method we get pellet which is protein and after centrifugation v have to collect the pellet and dissolve it into resuspension buffer.my problem is that my protein pellet is not getting fu...
by ss_kalve
Tue Jan 20, 2009 12:44 pm
Forum: Molecular Biology
Topic: Protein degradation
Replies: 5
Views: 5354

Protein degradation

can anybody tell that after how much time protein at room temperature will degrade? secondly, i've a doubt that after acetone precipitation i'm getting pellet which is not getting fully dissolved in resuspension buffer.what can be the reason and how can i dissolve the pellet of protein without any m...
by ss_kalve
Mon Jun 30, 2008 7:50 am
Forum: Molecular Biology
Topic: Problem in Tricine SDS PAGE
Replies: 0
Views: 1221

Problem in Tricine SDS PAGE

Hi, Can anybody tell that why we are getting such distorted band in 16%+6M urea tricine SDS PAGE?
by ss_kalve
Thu Jun 05, 2008 12:32 pm
Forum: Molecular Biology
Topic: lysosomal enzymes don't interfere with protein
Replies: 1
Views: 1981

lysosomal enzymes don't interfere with protein

Hi, can anyone tell in the process of protein extraction we crush the sample in mortar pastel. By this process lysosomal enzymes should come out and interfere with protein.so wat to do for that?
by ss_kalve
Wed Jun 04, 2008 5:02 am
Forum: Molecular Biology
Topic: primer design for DNA through mRNA sequence
Replies: 2
Views: 3652

primer design for DNA through mRNA sequence

Hi,
Can anybody tell that through availability of mRNA sequence can we design primer for genomic DNA. which kind of software we can use?
by ss_kalve
Thu May 29, 2008 12:21 pm
Forum: Molecular Biology
Topic: Lowery's method V/S Bradford's method
Replies: 3
Views: 10452

Lowery's method V/S Bradford's method

Hi,
plz anyone tell me for protein estimation which the best method Lowery's method or Bradford's method any why?