Search found 4 matches

by nexus2006
Tue Apr 08, 2008 5:09 pm
Forum: Molecular Biology
Topic: Filter particles out of insect cell lysate
Replies: 1
Views: 1409

Filter particles out of insect cell lysate

Hello,

i have an insect cell lysate with some large protein particles, which are ca. 20um x 3um, i like to separate from the rest debris swimming around. I thought about filtering cause centrifuging steps bring them down together.
Thank you very much for any ideas!
Regards,

Rudi
by nexus2006
Fri Dec 07, 2007 6:48 pm
Forum: Molecular Biology
Topic: baculovirus / insect cell / strange "crystals"?
Replies: 6
Views: 3920

TEM

Thanks anyway for your effort, i made now some TEM pictures and also from SDS-Page the structures appear to be protein crystals... now i am looking for a protocol to separate them from the cells :roll:
by nexus2006
Wed Nov 21, 2007 8:54 am
Forum: Molecular Biology
Topic: baculovirus / insect cell / strange "crystals"?
Replies: 6
Views: 3920

thank you very much for the inspiring hints! It is a AcMNPV encoding a protease which has a secretion signal. I also thought of crystals, protein or salt. we will do some TEM just for fun, to see if they are microorganisms or not. @biohazard: maybe one of your guys have seen those structures, i real...
by nexus2006
Tue Nov 20, 2007 9:53 am
Forum: Molecular Biology
Topic: baculovirus / insect cell / strange "crystals"?
Replies: 6
Views: 3920

baculovirus / insect cell / strange "crystals"?

Dear All, I have a question concerning the baculovirus expression system. I attached a picture of SF9-cell infected with my virus, it shows strange "needle"-like structure, they only appear in infected cells, i do controls with just the lipofection agent and the bacmid DNA alone. It looks ...