Search found 5 matches

by pluto
Thu Nov 15, 2007 7:03 am
Forum: Cell Biology
Topic: Effect of ethanol on proteins
Replies: 7
Views: 10520

yep... dehydrating = denaturing
by pluto
Thu Nov 15, 2007 6:58 am
Forum: Molecular Biology
Topic: extinction coefficient
Replies: 1
Views: 1615

can you just scrape it off, weigh it, and then measure an absorbance specturm? extinction coefficient is based on the absorance of a 1% solution.
by pluto
Thu Nov 15, 2007 6:57 am
Forum: Molecular Biology
Topic: about ATPS
Replies: 4
Views: 2130

to clarify on the post about ATP[gamma]S. When the gamma-P is replaced with an S, then that bond can no longer be cleaved (i.e. ATP->ADP). Thus, people use the "non hydrolyzable" form of nucleotides to study enzyme activity when you don't have to worry about substrate depletion
by pluto
Thu Nov 15, 2007 6:52 am
Forum: Molecular Biology
Topic: Digest
Replies: 7
Views: 3722

does it have star activity? use less enzyme next time if so.
by pluto
Thu Nov 15, 2007 6:52 am
Forum: Molecular Biology
Topic: Identifying Protiens
Replies: 2
Views: 1832

another way to do it is in silicia with prediction software