Search found 10 matches

by houlinlin
Sun Jul 06, 2008 7:41 am
Forum: Molecular Biology
Topic: ssDNA made by M13K07
Replies: 0
Views: 1308

ssDNA made by M13K07

Hi, has anyone here done the experiments about made ssDNA by M13K07?
I have done this experiment recently,but I couldnot get the ssDNA of my phagemid.
Could anyone tell me the reasons? Many thanks!
by houlinlin
Tue Apr 01, 2008 8:46 am
Forum: Molecular Biology
Topic: How to purify single-stranded cccDNA
Replies: 1
Views: 1139

How to purify single-stranded cccDNA

Hi,
does anyone know how to make single-strand cccDNA from double-strand plasmid? And how to purify?
Thanks a lot. :D
by houlinlin
Wed Mar 05, 2008 10:04 am
Forum: Molecular Biology
Topic: about Br-ATP
Replies: 1
Views: 1488

about Br-ATP

Hi,
I am confused by a kind of substance named Br-ATP.Could anyone show me the use and character of it ?
Thanks a lot. :)
by houlinlin
Sat Feb 23, 2008 9:00 am
Forum: Molecular Biology
Topic: unspecific co-immunoprecipitation
Replies: 1
Views: 1216

unspecific co-immunoprecipitation

HI,I am trapped in a experiment about checking unspecific co-immunoprecipitation.Could anyone help me ? Thanks.
by houlinlin
Thu Dec 20, 2007 1:50 am
Forum: Molecular Biology
Topic: protein interaction
Replies: 4
Views: 2919

protein interaction

HELP! :?:
Does anyone know the methods which can detect the interaction between proteins the most accurately?
by houlinlin
Wed Nov 07, 2007 2:03 am
Forum: Molecular Biology
Topic: SSV1
Replies: 0
Views: 828

SSV1

Is SSV1 a double-stranded virus or a single-stranded one? :(
by houlinlin
Tue Nov 06, 2007 1:40 am
Forum: Molecular Biology
Topic: About 'spurious stop'
Replies: 0
Views: 1352

About 'spurious stop'

There is a sentence described like this ' SsXpb-II showed a single start site mapping at -4 from the first ATG of the ORF, and a site internal to the ORF (+10), likely due to a spurious stop of reverse transcriptase. ' in an article named'Transcriptional response to DNA damage in the archaeon Sulfol...
by houlinlin
Mon Nov 05, 2007 12:43 am
Forum: Molecular Biology
Topic: about ATPS
Replies: 4
Views: 2066

Thanks for your replys.I am sure I have got the answer.
by houlinlin
Sun Nov 04, 2007 2:04 am
Forum: Molecular Biology
Topic: about ATPS
Replies: 4
Views: 2066

about ATPS

I am confused by the differences of ATP and ATPS appeared in an article.would anyone show me what are the differences between them ?
Thanks very much! :)
by houlinlin
Wed Oct 31, 2007 9:38 am
Forum: Molecular Biology
Topic: genome shuffling
Replies: 1
Views: 1674

genome shuffling

Would anyone tell what is the principle of 'genome shuffling'? :shock: