Search found 2 matches

by ll2013
Mon Jun 17, 2013 1:51 pm
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 5777

Thanks for all of your help! I did more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, instead of using microwave, this time I used a heating block with stirring. 3. Used a much bigger el...
by ll2013
Thu Jun 06, 2013 1:09 am
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 5777

Asking for help with electrophoresis

Dear all, my lab has some problems with DNA agarose gel electrophoresis. First, the small DNAs cannot be separated. We are using BioLine hyperladder II as marker, which has bands from 50bp to 2000bp. The separation from 300bp to 2000bp is just fine. However, the 50bp, 100bp, 200bp and 300bp bands ha...