Search found 9 matches

by Shaanu
Sun May 11, 2014 11:01 am
Forum: Genetics
Topic: Mutagenesis
Replies: 2
Views: 3950

Re: Mutagenesis

???
No one knows .. OOps? :( :(
by Shaanu
Wed May 07, 2014 8:23 pm
Forum: Genetics
Topic: Mutagenesis
Replies: 2
Views: 3950

Mutagenesis

Halo :) Can anyone explain in simple words what is en passant mutagenesis. How it
is different from usual methods of manipulating genomes.
by Shaanu
Mon Jun 17, 2013 9:04 am
Forum: Cell Biology
Topic: Cell Signaling
Replies: 1
Views: 2779

Cell Signaling

When one studies the protein protein interaction, why it is sometimes recommended to perform in vitro linked transcription translation, when immunoprecipitation anyhow confirms your in vivo results. I have done this in my masters thesis but have been asked by many that what is the objective. I could...
by Shaanu
Sat Jun 08, 2013 3:53 pm
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 4565

Dear Jonmoulton.The pull down certainly gives information on protein protein interaction [as done with the Beads to pull down.] I am not sure whether it made sense or sounds weird to you ::)
by Shaanu
Mon Jun 03, 2013 7:12 am
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 4565

In Vitro Transcription Translation

Hi . I have recently started working with in vitro linked transcriotion:translation to prove my in vivo results of interactions between two proteins. I used rabbit reticulocytes system and analysed the pull down on commassie gel with S-35 already added in reaction and autoradiographed to visulalize...
by Shaanu
Fri May 10, 2013 1:59 pm
Forum: Molecular Biology
Topic: Stem Cells
Replies: 4
Views: 5006

Re: Stem Cells

Thanks !
by Shaanu
Fri May 10, 2013 8:10 am
Forum: Molecular Biology
Topic: Stem Cells
Replies: 4
Views: 5006

Stem Cells

How can one prove in vivo that a cell is a stem cell ? Can anyone give hints citing any two methods.
by Shaanu
Thu May 09, 2013 3:00 pm
Forum: Molecular Biology
Topic: Stem Cells
Replies: 0
Views: 2892

Stem Cells

I am an undergraduate student who recently appeared for Hannover Medical School Phd Entrance examination. They asked many questions to test analytical abilities one of which was" How will you prove in vivo that a cell is stem cell" ? Though I have very less knowledge in this field I gave t...
by Shaanu
Thu May 09, 2013 9:45 am
Forum: Genetics
Topic: Stem Cells
Replies: 5
Views: 13637

Stem Cells

I am an undergraduate student who recently appeared for Hannover Medical School Phd Entrance examination. They asked many questions to test analytical abilities one of which was" How will you prove in vivo that a cell is stem cell" ? Though I have very less knowledge in this field I gave t...