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by seanyumcloughlin
Sun Sep 02, 2007 11:16 pm
Forum: Molecular Biology
Topic: Primers for QuikChange - desalted vs PAGE purified
Replies: 2
Views: 2920

Primers for QuikChange - desalted vs PAGE purified

Hi Everyone, I'm new here, nice site, nice to be here. I'm in a new lab where they use short (30 nt), desalted primers to do the QuikChange rxn. Stratagene recommmends HPLC or PAGE purified primers. Using ordinary primers, I get a nice yield of PCR product, but very few transformants. I used to use ...