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I realise that each of the 12 primer pairs bind to a different region of human genome; the VNTR's within each region, and that is how 12 distinct bands can be detected after PCR. But what i don't know is why the exact same primers that target the same location end up a different areas.
- Mon Jan 28, 2013 12:25 pm
- Forum: Off Topic Discussion
- Topic: Which is best? Biology or Chemistry or Physics
- Replies: 20
- Views: 90879
Well definitely not physics xD
Can someone tell me why a mixture of 12 specific primers produces 12 bands from a single persons DNA. And why is the pattern of bands produced by multiplex PCR with the same 12 primers highly variable between individuals? What are the most likely explanations for individual D having less than 12 ban...