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by Nina
Mon Aug 06, 2007 10:57 am
Forum: Molecular Biology
Topic: PCR with labeled primers - help
Replies: 0
Views: 957

PCR with labeled primers - help

I have optimized PCR reactions with non-labeled primers, when I perform the reaction with FAM or NED labeled primers sometimes it is OK but in few cases I can't amplify anything although the non labelled primers work. Any ideas how to solve this?
Thanks in advance!