Search found 10 matches

by Bio2013
Sat Mar 16, 2013 5:12 pm
Forum: Molecular Biology
Topic: dot blot
Replies: 3
Views: 3198

I am having problems with that. I am using Nitrocellulose membrane, at the beginning I was getting bleeding and leakage between the wells, next I saw shadow around the dots and now I am getting no signal.
any similar experience?
by Bio2013
Thu Mar 14, 2013 6:07 am
Forum: Molecular Biology
Topic: dot blot
Replies: 3
Views: 3198

dot blot

Does any body have any experience with ''dot blot''?
http://www.bio-rad.com/prd/en/US/LSR/PD ... -Apparatus
thanks
by Bio2013
Sat Feb 16, 2013 8:50 pm
Forum: Molecular Biology
Topic: ELISA
Replies: 1
Views: 2477

ELISA

Does anyone have a protocol for ELISA?
Thanks
by Bio2013
Sat Feb 16, 2013 8:48 pm
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 7711

Re:

JackBean wrote:Those affecting size on SDS-PAGE would be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?


sorry, but I do not know what MALSI MS is.
by Bio2013
Sat Feb 16, 2013 8:48 pm
Forum: Molecular Biology
Topic: seahorse
Replies: 3
Views: 3370

Re:

JackBean wrote:Seahorse is the animal, isn't it?


Actually, it is an animal, but its alo the name of a biological apparatus, which I dont have experience with.
by Bio2013
Mon Feb 11, 2013 6:17 am
Forum: Molecular Biology
Topic: seahorse
Replies: 3
Views: 3370

seahorse

hello,
does anyone know how seahorse work? what are its applications? thanks
by Bio2013
Mon Feb 11, 2013 5:26 am
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 7711

Re:

What about post-transcrioptional modifications? Actually, I do not know exactly how to measure post translational modifications and reduce them from the full size of the protein, can any body help me with that? would post translational modifications be same for all proteins, and how can I find out ...
by Bio2013
Tue Feb 05, 2013 11:43 pm
Forum: Molecular Biology
Topic: HPLC
Replies: 1
Views: 1488

HPLC

Hi,
Does anybody know how much water do I need to add to my cells after counting them for HPLC?

Thanks
by Bio2013
Thu Jan 31, 2013 7:48 pm
Forum: Molecular Biology
Topic: thaw cells
Replies: 1
Views: 1672

thaw cells

Does anyone have areliable protocol for thawing cells from frozen stock?
thanks
by Bio2013
Wed Jan 30, 2013 7:00 am
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 7711

Western Blot

Hi BIologists,
I have a question, how can we know about the size of protein in the western blot? I know the full lenght, but after WB, I am seeing band with a different size!
Thanks for your help.