Search found 2 matches

by tohelpandtobehelped
Sat Dec 29, 2012 3:46 pm
Forum: Molecular Biology
Topic: Blue and White colony sreening
Replies: 2
Views: 2947

If I have not been specific enough or maybe to broad... Please tell me and i will do my best to make it clearer. I have searched for quite a while but its all about the differences between the two and not the absence...
by tohelpandtobehelped
Sat Dec 29, 2012 3:43 pm
Forum: Molecular Biology
Topic: Blue and White colony sreening
Replies: 2
Views: 2947

Blue and White colony sreening

For colony PCR reactions white colonies were picked from the transformation plate. I performed transformation of cloned ANO5 cDNA and the transformation mixture was plated on a LB agar plate containing ampicillin +IPTG+X-Gal. The ANO5 cDNA was cloned in the expression vector pdEYFPC1amp. No blue col...