Search found 2 matches

by gothica
Sat Jul 14, 2007 12:52 pm
Forum: Molecular Biology
Topic: RNA extraction Kit vs Trizol
Replies: 7
Views: 7847

Uupss, I am so sorry. I couldn't realize the application site. Now I change my comments. It should be DNA contamination. You can apply DNase tratment and then run again. I had same problem one times and ı repeated DNase treatment step only. It worked.

MSc student
METU (Turkey)
by gothica
Sat Jul 14, 2007 12:48 pm
Forum: Molecular Biology
Topic: RNA extraction Kit vs Trizol
Replies: 7
Views: 7847

Hi, Actuallay if it is a mamalian sample we should see 3 RNA bands (rRNA bands) if your sample is taken from plant we expect to see four bands. In your gel we saw three bands isolated by kit and 2 bands isolated by trizol. To me it isn't DNA contamination. If it was DNA it had to run slower than RNA...