Search found 7 matches

by hurzz
Mon Nov 26, 2012 3:40 am
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

Re: How can a protein without a G-site bind to GSH?

Thank you very much, pretty much what i thought as well. i have to find a facility where i can do N-term sequencing. we don't have it here. i have abt 1.5mg of protein, ran on SDS-PAGE got a single band, but when i did 2DE i got 2 spots of the same MW but different pI. sent it for MALDI-ToF-ToF, thi...
by hurzz
Sun Nov 25, 2012 7:37 am
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

species the sample from is Tachypleus gigas.
by hurzz
Sun Nov 25, 2012 7:36 am
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

yes 3% by maldi-tof. my species is horseshoe crab. i suspect the protein is not in database. do you think i should proceed with N-terminal sequence for whole protein sequence then?
by hurzz
Thu Nov 22, 2012 5:52 pm
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

I re-post this, just in case you didn't receive my PM. Thank you for your time. 1 MIDETAYIHP SAIVEDGAVI GANVRIGPFC CIGSQVEIGE GTELKSHVVV NGITKIGRDN QIFQFASIGE MNQDLKYHGE 81 PTRVEIGDRN RIRESVTIHR GTVQGGGVTK IGNDNLLMVN AHIAHDCIVG DRCVIANNGT LGGHVILGDY VIIGGMSAIH 161 QFCQIGSHAM VGGCSGVVQD IPPYVIAQGN HA...
by hurzz
Thu Nov 22, 2012 12:51 pm
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

i have PM u. Thank you very much.
by hurzz
Thu Nov 22, 2012 11:19 am
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

Re: How can a protein without a G-site bind to GSH?

Hello Mr JackBean

yes i only got 1 band and the score for that 3% sequence is 100% of an acyl carrier protein lpxA-like motive.
my suspicion is this animal utilises an analog of GSH.

Thank you very much.
by hurzz
Mon Nov 12, 2012 12:34 pm
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 7720

How can a protein without a G-site bind to GSH?

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. question: 1. how can my protei...