Search found 3 matches

by icequeen11
Fri Jun 15, 2007 7:15 pm
Forum: Molecular Biology
Topic: Primary lambda phage screen - precipitated probe?
Replies: 1
Views: 2495

Primary lambda phage screen - precipitated probe?

Hey all, I just performed a primary screen of a genomic library. My films look spotty, but not in the same mottled way as the plated phage plaques, more random. I did duplicate lifts, and while both films are spotty to the same degree, few (if any) of the spots overlap. Going back to the procedure, ...
by icequeen11
Fri Jun 15, 2007 6:53 pm
Forum: Molecular Biology
Topic: Precaution steps for casting a gel.
Replies: 4
Views: 6258

Let the agarose cool till you can touch the flask with your bare hand, pain-free, before adding the EtBr. Swirl the flask every now and then as it's cooling too, to avoid getting polymerizing regions against the glass. I was doing Southern blots; I don't know how important it is for normal check gel...
by icequeen11
Tue May 22, 2007 7:43 pm
Forum: Molecular Biology
Topic: Smeary restriction digest of genomic DNA
Replies: 1
Views: 2458

Smeary restriction digest of genomic DNA

Hey all, same prob as a recent post, but working with genomic preps (from fish muscle tisue) instead of plasmids. I ran a digest with a single enzyme (Sac I) on 8 different samples, and did an incubation with the specified buffer as a control for each sample. The same tubes of BSA and buffer were us...