Search found 16 matches

by triton
Thu Sep 13, 2012 11:12 am
Forum: Molecular Biology
Topic: DNA extraction from Human blood
Replies: 3
Views: 4610

DNA looks good. There is some problem with electrophoresis. Probably you overloaded (too much DNA) and thus the wierd appearance of your bands. Try to load little bid less of sample and use lower voltage. stain the gel after electrophoresis, not before.
by triton
Thu Sep 13, 2012 11:05 am
Forum: Molecular Biology
Topic: RT PCR contamination.
Replies: 2
Views: 7507

what is an organism you are working with? is it present everywhere, like bacteria, or do you work with something rare like endemic mice of Madagascar? Did you consider contamination of your pipets? Do you use tips with filters?
by triton
Thu Sep 13, 2012 10:58 am
Forum: Microbiology
Topic: Viruses VS Bacteria
Replies: 4
Views: 8495

Re: Viruses VS Bacteria

According to what I heard- viruses are considered as living organisms. Living organisms are now divided into 2 clusters: 1-those that does not encode ribosomes (viruses) 2-those that encode ribosomes (everything else)
by triton
Mon Aug 27, 2012 4:46 pm
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 8640

1-are you sure you have an insert in your vector? If not, there is a big chance the vector is empty and therefore you have no desired band. PCR verify presence of insert in vector. 2-something wrong with restriction reaction (verify if your enzymes work on different sample) 3-there is a methylation ...
by triton
Sat Aug 25, 2012 8:46 am
Forum: Molecular Biology
Topic: isolation of plasmid (Midi prep)
Replies: 3
Views: 8640

Im little bit confused, but if I unserstand it correctly-restriction reaction does not produce desired cut? I do not know what you do. First thing is to check if the desired product is in your plasmid. Do PCR reaction. The second possibility is that the band size does not match theroretical due to e...
by triton
Wed Aug 22, 2012 7:40 pm
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 9281

you can download ncbi database into your computer, install command line blast and do your search in you computer. You can have as many sequences as your processor tolerate. alternatively you can create an account in BIOPORTAL OSLO and use their cluster for blasting. You can submit milions of sequenc...
by triton
Wed Aug 22, 2012 7:34 pm
Forum: Molecular Biology
Topic: Vector
Replies: 2
Views: 3019

I dont get it. What do you mean by MASS??? If the total amount i.e. weight, so simply multiply your concentration by the total volume...
by triton
Wed Aug 22, 2012 7:27 pm
Forum: Molecular Biology
Topic: pcr purification trouble
Replies: 4
Views: 6468

Re: pcr purification trouble

Hello all I am into a trouble need some suggestion to correct myself. I had my pcr and I used 2 step pcr and i observed the band on agarose my desired sequence. Measured the concentration before and after pcr purification, but the concentration got down considerbaly. I did not purify the whole amou...
by triton
Wed Aug 22, 2012 7:12 pm
Forum: Microbiology
Topic: Salmonela E-coli and others - for experts only
Replies: 10
Views: 6869

yes bacteria generally survive freezing, but it is better to preserve them in 10-20% glycerol and yes they survive in the stomach
by triton
Wed Aug 22, 2012 7:03 pm
Forum: Microbiology
Topic: Microbiology help in identifying Gram + rod for my unknown
Replies: 12
Views: 16999

Re:

JackBean wrote:My guess would be they have it for some school labs, where it is the purpose to try all these tests and identify the bacteria.


OH, its obvious :D Im such an idiot
by triton
Wed Aug 22, 2012 7:01 pm
Forum: Microbiology
Topic: Microorganisms and organic preservation?
Replies: 1
Views: 7232

Hi, I'm not sure if I understand what you want but, did you try simply change the water for organic solvent that will evaporate? Fix the fruit with protein linking fixative like 2% paraformaldehyde or glutaraldehyde for several days. Put the fixed fruit into serie of organic solvent concentration gr...
by triton
Wed Aug 22, 2012 11:53 am
Forum: Microbiology
Topic: Black coloration of sheep blood agar
Replies: 3
Views: 12447

Re:

Hmmh... as far as I can tell that looks quite normal non-hemolytic growth typical to Bacillus species, though the angle and light are not ideal for spotting it. I am not sure what causes the darker coloration of the agar (I'd call it deep red rather than black from what I see), but it is a common p...