Search found 1 match
Search found 1 match • Page 1 of 1
- Wed Apr 18, 2007 10:13 pm
- Forum: Molecular Biology
- Topic: Does gene subcloning affect protein activity?
- Replies: 2
- Views: 1688
With its protein not expressing well in the original vector, I subcloned my gene of interest into another plasmid. Although I saw an improvement in the protein expression level for both the wild-type and mutant proteins, the activity of these proteins was more than 10 fold lower compared to the prev...