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by chopin1810sy
Wed Apr 18, 2007 10:13 pm
Forum: Molecular Biology
Topic: Does gene subcloning affect protein activity?
Replies: 2
Views: 1688

Does gene subcloning affect protein activity?

With its protein not expressing well in the original vector, I subcloned my gene of interest into another plasmid. Although I saw an improvement in the protein expression level for both the wild-type and mutant proteins, the activity of these proteins was more than 10 fold lower compared to the prev...