Search found 2 matches

by jasfire
Tue Apr 17, 2007 3:56 pm
Forum: Molecular Biology
Topic: problem in GST pulldown
Replies: 3
Views: 2337

the S is out-of-date(about 2 month),but the free isotope could be detected.Does that mean the isotope still can work?
by jasfire
Tue Apr 10, 2007 3:50 pm
Forum: Molecular Biology
Topic: problem in GST pulldown
Replies: 3
Views: 2337

problem in GST pulldown

Hi,guys I'm now doing GST-pulldown to detect the interaction between two known protein.But there's no binding detected. One of my proteins is fused with GST,and expressed in E.coli which is sonicated to gain the protein;the other is translated labeled with S35-Met in vitro in TnT system.I'm sure the...