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- Tue Apr 10, 2007 3:50 pm
- Forum: Molecular Biology
- Topic: problem in GST pulldown
- Replies: 3
- Views: 2337
Hi,guys I'm now doing GST-pulldown to detect the interaction between two known protein.But there's no binding detected. One of my proteins is fused with GST,and expressed in E.coli which is sonicated to gain the protein;the other is translated labeled with S35-Met in vitro in TnT system.I'm sure the...