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- Tue Mar 12, 2013 8:04 pm
- Forum: Molecular Biology
- Topic: How can I add a kozak sequence in plasmid pMT/bip/V5-His A
- Replies: 1
- Views: 4027
Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? S...