Search found 1 match

by yiwi123
Tue Mar 12, 2013 8:04 pm
Forum: Molecular Biology
Topic: How can I add a kozak sequence in plasmid pMT/bip/V5-His A
Replies: 1
Views: 3985

How can I add a kozak sequence in plasmid pMT/bip/V5-His A

Hi guys, I'm a new guy in molecular biology. I plan to express a scfv in S2 cell using the plasmid pMT/bip/V5-His A (with enzyme site BglII and XbaI). Because there's a Bip signal sequence ahead of the enzyme site BglII, so I CAN NOT add Kozak sequence in the N-Term of my scfv gene, is that right? S...