Search found 8 matches

by elynn
Tue Sep 09, 2014 6:48 am
Forum: Molecular Biology
Topic: exon intron junction
Replies: 0
Views: 2597

exon intron junction

Hi people! Please help me. Do you guys know other software that can predict exon-intron junction apart from GENESCAN, NetGene, FGENESH and RNASPL? I also had tried multiple sequence alignment but I need other software to strengthen my result. Unfortunately the software above are not enough. Thank yo...
by elynn
Thu Dec 20, 2012 2:48 am
Forum: Molecular Biology
Topic: How to use PCR enhancer
Replies: 1
Views: 1669

How to use PCR enhancer

Hi,
I would like to know how to add PCR enhancer.. From my reading, it says that i can use 6.7% DMSO or 1.25% Formamide. but i'm confuse the % is from the total PCR reaction or from the concentration of stock. Do you guys have any idea?

Thank you.
by elynn
Wed Dec 05, 2012 9:12 am
Forum: Molecular Biology
Topic: PCR not consistent
Replies: 3
Views: 3696

PCR not consistent

Dear everyone, I have a problem with my PCR. Its not consistent. Sometimes i got bands on my gel and sometimes i don't get bands. however the mixture and pcr protocol is the same. I hv check my DNA and primers and they're in good conditions. Is it normal to have this kind of problem? Can anyone sugg...
by elynn
Mon Oct 01, 2012 4:46 am
Forum: Molecular Biology
Topic: Nucleotide Database
Replies: 9
Views: 6445

APETALA is not only known in Arabidopsis.. it is a flowering gene..so it should be presented in angiosperm.. and i hv few sequence of apetala from other species..unfortunately its not enough to design a good primer
by elynn
Wed Sep 19, 2012 2:13 pm
Forum: Molecular Biology
Topic: Nucleotide Database
Replies: 9
Views: 6445

APETALA gene from hibiscus sabdariffa. and i dont know the gene sequence..thats why i need to design a pair of primer to use it for isolating full length cDNA
by elynn
Wed Sep 19, 2012 1:59 pm
Forum: Molecular Biology
Topic: Nucleotide Database
Replies: 9
Views: 6445

Nucleotide Database

I am trying to design a good primer for isolating my target genes..unfortunately the sequence references are very limited..most of the database lead me to arabidopsis..n i dont want to only use arabidopsis..i wanna isolate apetala genes but due to the limited sequences, i cant design a good primer.....
by elynn
Mon Jun 18, 2012 11:45 am
Forum: Molecular Biology
Topic: PCR enhancer
Replies: 0
Views: 1236

PCR enhancer

How to use PCR enhancer? N how to determine which enhancer i need to use?
by elynn
Wed Jun 06, 2012 5:29 pm
Forum: Molecular Biology
Topic: PCR master mix
Replies: 1
Views: 2394

PCR master mix

what is the purpose of every chemical use in the master mix?
n how to optimize the pcr reaction?
please help me