Search found 19 matches

by nukeman
Wed May 15, 2013 11:33 am
Forum: Molecular Biology
Topic: Use of cDNA when studying splicing
Replies: 2
Views: 8747

Use of cDNA when studying splicing

Hi I've read a few studies where the authors aim to study the association of certain proteins to mRNA in a splicing-dependent manner. Mainly they compared samples containing spliced mRNA with cDNA-samples, in a way that to me seemed as if they insinuated that the cDNA samples represented the non-spl...
by nukeman
Wed May 15, 2013 11:29 am
Forum: Molecular Biology
Topic: What are 'interchromatin granule clusters'?
Replies: 2
Views: 2780

A bit late with the thanks, but still, thank you.
by nukeman
Thu Mar 28, 2013 2:23 pm
Forum: Molecular Biology
Topic: What are 'interchromatin granule clusters'?
Replies: 2
Views: 2780

What are 'interchromatin granule clusters'?

Hi,

I've been looking around for a more detailed description of these things but I just can't find anything useful. Quite simply, I just want to know what interchromatin granule clusters are, and if possible, a rough overview of composition and/or function.

Cheers
by nukeman
Thu Mar 28, 2013 2:21 pm
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 23956

Cis and trans-acting regulatory elements are in essence transcriptional regulators/promoter sequences that are categorized based on acting distance. The lac operon in E.coli is a typical example of a cis-acting element. Promoter sequences that regulate expression of the genes contained within the op...
by nukeman
Mon Jan 14, 2013 3:47 pm
Forum: Microbiology
Topic: Difference between Virulence and Pathogenicity?
Replies: 1
Views: 4919

Difference between Virulence and Pathogenicity?

Greetings,

I have read several text regarding this, but I still can't manage do discriminate between them. So far what I've got is: Pathogenicity = The potential/ability of a infectious agent to cause damage. Virulence = The actual level of damage caused.

Is this correct or am I mistaken?
by nukeman
Mon Dec 03, 2012 11:57 am
Forum: Molecular Biology
Topic: Mechanism of Saponin in laboratory use?
Replies: 0
Views: 2259

Mechanism of Saponin in laboratory use?

Greetings, I was googling around regarding the use of Saponin in the lab, but I couldn't really find anything useful. As far as I know, it's used to get stuff inside the cells, but I can't figure out whether it's a regular detergent or some kind of vesicle forming compound that 'transports' whatever...
by nukeman
Wed Oct 31, 2012 5:21 pm
Forum: Molecular Biology
Topic: Direct or indirect protein-DNA interaction
Replies: 1
Views: 2139

Direct or indirect protein-DNA interaction

Greetings, How would one go about deducing whether a certain DNA binding transcription factor has a direct interaction with a gene of interest ( we know that the TF has impact on expression because a mutation in the gene coding for it caused a 10-fold decrease in expression of my gene of interest), ...
by nukeman
Mon Sep 17, 2012 9:48 am
Forum: Molecular Biology
Topic: Synthetitic genetic screening in yeast
Replies: 4
Views: 6699

Re: Synthetitic genetic screening in yeast

I understand, thank you very much for the clarifications.
by nukeman
Tue Sep 11, 2012 4:22 pm
Forum: Molecular Biology
Topic: Synthetitic genetic screening in yeast
Replies: 4
Views: 6699

I think I see what you mean. I talked with my teacher briefly today, and he said that simply what you do is, you run for example 100 genes. You start by mutating gene 1 and then mutating gene 2, then you proceed with mutating gene 1 again, but this time you run it with a mutated gene 3. If you know ...
by nukeman
Mon Sep 10, 2012 5:57 pm
Forum: Molecular Biology
Topic: Synthetitic genetic screening in yeast
Replies: 4
Views: 6699

Synthetitic genetic screening in yeast

Hi, The concept of Synthetic-lethality and Synthetic Genetic Array have been introduced to us in class recently, but I'm struggling with understanding it. Quite frankly, I don't even understand what questions one is seeking answer to using these techniques. I was given some papers to read up on so t...
by nukeman
Fri Jun 01, 2012 2:14 pm
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 15583

Yeah, I did something like that. Thought I'd look around what other options were available, never hurts to know more :P

Anyways, thank you very much for your help :)
by nukeman
Thu May 31, 2012 4:52 pm
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 15583

I am aware of that - luckily I'm allowed to modify the question the way I want. :P It's not so much that we should get a "right/wrong" answer, it's more about the teacher evaluating the use of our knowledge and methodical approach.