Search found 9 matches

by operon
Wed Jan 28, 2009 1:54 am
Forum: General Discussion
Topic: Interested in pursuing research in gene therapy
Replies: 3
Views: 2017

Interested in pursuing research in gene therapy

Hi I'm doing my final year for my B.Sc in cell/molecular biology and I want to focus my graduate life on gene therapy. How does someone go about doing this since there are no real programs that specifically deal with gene therapy? Should I try to get into medical school, should I apply into a geneti...
by operon
Sat Aug 09, 2008 6:11 am
Forum: Molecular Biology
Topic: Primers not working
Replies: 7
Views: 3952

I'm an undergraduate and want to understand. What is this vector NTI program? Designing primers sounds fun but how do you do it? I guess you find out the flanking sequences of the gene and use these to make the primer? How do you make the primer? Where do you find the sequence information in desigin...
by operon
Sun Apr 15, 2007 5:37 am
Forum: Molecular Biology
Topic: Two questions, mobility shift, primer extension analysis
Replies: 5
Views: 2655

UGH. I did it again. I meant an OVERLAPPING promoter. Not a reverse promoter, not a reverse primer. ANYWAYS...I think the alternative splicing is the best answer. Thanks a lot!
by operon
Thu Apr 12, 2007 12:32 am
Forum: Molecular Biology
Topic: Two questions, mobility shift, primer extension analysis
Replies: 5
Views: 2655

For question 1. Would it also be possible that another promoter lies just upstream and the primer extends to this one as well? In my question I foolishly mentioned a reverse primer. What I meant what a reverse promoter (I'm not sure I would understand why this might give some insight, I just remembe...
by operon
Sun Apr 08, 2007 4:40 pm
Forum: Molecular Biology
Topic: Two questions, mobility shift, primer extension analysis
Replies: 5
Views: 2655

Two questions, mobility shift, primer extension analysis

Hello again duders! I have two questions here (they are attached as JPEGS), and I was wondering if some of you could help me. For the first question I got 6/10, for the second I got 7/10. So I was hoping you guys could help clarify what is missing! Okay, question 1 (see img002.jpg). a) What can you ...
by operon
Mon Mar 12, 2007 9:17 pm
Forum: Molecular Biology
Topic: Questions regarding lac/trp operons
Replies: 2
Views: 6384

Okay, forget that, consider this I know that in the presence of glucose ALONE we still get negligible lac products (let's say 0.5 units) because there is always some allolactose present (this is the inducer, it binds to the repressor and gets off the operator, thus allowing transcription) NOW, in an...
by operon
Sat Mar 10, 2007 6:00 pm
Forum: Molecular Biology
Topic: Questions regarding lac/trp operons
Replies: 2
Views: 6384

Hi again!

Is there anyone who can help me? I have a quiz on Tuesday and I need to know if what I'm doing is indeed the correct method.
by operon
Thu Mar 08, 2007 8:07 pm
Forum: Molecular Biology
Topic: gene copy numbers
Replies: 4
Views: 2287

My understanding of it is that all humans (and probably most vertebrates?) have in that DNA specific repeated sequences. These repeats, I believe, arose when a certain part of the DNA was cut out(let's call it A) and then inserted at another part (let's say two units indicated as - -) so then you'd ...
by operon
Thu Mar 08, 2007 7:32 pm
Forum: Molecular Biology
Topic: Questions regarding lac/trp operons
Replies: 2
Views: 6384

Questions regarding lac/trp operons

Hi. I'm in a molecular biology class and I was wondering if some of you guys can verify some of my answers to two questions: 1. Wild type E. coli expresses 05 units of B-gal when grown in glucose medium and 1500 units when grown in lactose medium. How much B-gal would you expect in each of the follo...