Search found 6 matches

by LittleBird
Sun May 20, 2012 5:55 pm
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 7612

Hi,

as far as I know expressed intracellulary and then secreted into the medium.

The medium is casual DMEM (http://de-de.invitrogen.com/site/de/de/ ... n.170.html), with high glucose and L-Glutamin with 5% fetal calf serum and 5% penicillin/streptomycin.

Or do you mean anything else?
by LittleBird
Sun May 20, 2012 3:43 pm
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 7612

Why dialyse DMEM before protein purification (Ni-Affinity)?

Hey there, the question may seem dumb at first sight, I hope all the same it's not too stupid to ask.... It can be reduced to "why is it - in this case - necessary to perform a dialysis?" We transfected HEK293T in order to express a his-tagged protein. The cells were kept in DMEM (high glu...
by LittleBird
Sun May 20, 2012 3:16 pm
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 623920

Re: Introduction for members

My appreciation! I perceive German as a rather difficult language to learn. Sometimes even I (as a native speaker) struggle to choose the appropriate expression (e.g. there are some terms containing either 'mich' or 'mir' and every now and then I hesitate in order to estimate which one of them is th...
by LittleBird
Thu May 17, 2012 11:18 am
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 623920

*laughs* Yes, indeed, show-off was what I wanted to say (of course in a kind way ;))
You're learning german?
by LittleBird
Thu May 17, 2012 10:37 am
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 623920

Re: Introduction for members

Angeber :wink:
Ich komme aus Franken (Bayern) und besuche in Erlangen die Universit├Ąt.
by LittleBird
Wed May 16, 2012 8:04 pm
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 623920

Hey Guys! Name: Lilly Nationality: German Gender: XX Age: 22 Education: Studying at the university. If everything works out fine, I'll be a BSc. soon. Interest: I must admit I cannot write sth really extraordinary in here, for I guess I'm a rather casual girl: I listen to a lot of music, I like moun...