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by lkmcneil
Mon Feb 12, 2007 1:44 pm
Forum: Molecular Biology
Topic: ratios for ligation reactions
Replies: 2
Views: 2197

ligation ratio

The insert should be present at a molar ratio of 3 to every 1 plasmid. If your plasmid is about 3kb and your insert is about 1 kb, then just use equal masses of each; 250 ng vector and 250 ng insert.