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by sergii
Wed May 02, 2012 7:32 pm
Forum: Molecular Biology
Topic: How to prove that thiol groups are de-protected?
Replies: 2
Views: 2937

How to prove that thiol groups are de-protected?

Hi everyone. I'm trying to link chemically a protein with a DNA oligonucleotide. I think one of the simplest way would be to use Michael addition, where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonuc...