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- Wed May 02, 2012 7:32 pm
- Forum: Molecular Biology
- Topic: How to prove that thiol groups are de-protected?
- Replies: 2
- Views: 2883
Hi everyone. I'm trying to link chemically a protein with a DNA oligonucleotide. I think one of the simplest way would be to use Michael addition, where thiol group reacts with C=C electron deficient bond. Befor trying to work with proteins, which could be hard, I'm trying to ligate two DNA oligonuc...