Search found 3 matches

by epsilonbeta
Wed Feb 07, 2007 7:55 pm
Forum: Molecular Biology
Topic: PCR primers
Replies: 2
Views: 1484

e pcr

You can do e-PCR ...another alternative...
link:

http://www.ncbi.nlm.nih.gov/sutils/e-pc ... m=0&gaps=0


put yr forward and reverse primer..and the expected size... and you should get a hit with it...
by epsilonbeta
Tue Feb 06, 2007 11:47 am
Forum: Molecular Biology
Topic: RNA Primer
Replies: 3
Views: 6240

RNA primers are synthesized by PRIMOSOME, specifically, dnaG or primase enzyme... the RNA primer came from dnaG plus dna B interactions....so I guess your question should be put in another way... the RNA primer is the product, yet the PRIMOSOME is the one that decides where primers are synthesized.....
by epsilonbeta
Fri Feb 02, 2007 7:50 am
Forum: Molecular Biology
Topic: Screening normal DNA for a silent mutation foundIn a patient
Replies: 2
Views: 2744

Screening normal DNA for a silent mutation foundIn a patient

We have a patient with a silent mutation, but the sequence change does not create/abolish any restriction enzyme site. We tried allele specific PCR, to check if the silent mutation is present in the normal population, but my student is having a hard time with the optimisation. Its not that she's doi...