Search found 4 matches

by foufoulette
Fri Apr 13, 2012 2:01 am
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 3615

I made transformation and i had colonies but what do you mean by yield ?
by foufoulette
Thu Apr 12, 2012 12:01 am
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 3615

Ok i understand but actually I had inactivated phosphatase in 65°C after ligation and it's correct i think, now i'm waiting for the results !
by foufoulette
Wed Apr 11, 2012 12:35 pm
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 3615

Re: Inactivate an enzyme

The enzyme could do something if the DNA insert is not dephosphorylased, so what shall i do ? cause i don't need to dephosphorylate the insert.
by foufoulette
Wed Apr 11, 2012 1:53 am
Forum: Molecular Biology
Topic: Inactivate an enzyme
Replies: 7
Views: 3615

Inactivate an enzyme

Hi everyone !

Please, i need your opignon about what i've done. Is it correct to don't inactivate enzyme (phosphatase) after a diphosphatasing of a plasmid and do the next step which is ligation, without inactivation ? is this could affect my results ?