Search found 16 matches

by Sue4
Tue Aug 26, 2014 3:27 am
Forum: Molecular Biology
Topic: B-DNA and A-DNA
Replies: 4
Views: 7860

Thank you.. But B-DNA is the normal Watson Crick double helix form of DNA isn't it? So it should be the one discovered first right?
by Sue4
Mon Aug 25, 2014 10:06 am
Forum: Molecular Biology
Topic: B-DNA and A-DNA
Replies: 4
Views: 7860

B-DNA and A-DNA

What does B in B-DNA stand for? And A in A-DNA? I couldn't find any answer by searching.I'm thankful if you can explain it to me. Thank you
:shock:
by Sue4
Sun Mar 02, 2014 10:07 am
Forum: Cell Biology
Topic: Why can't sperm cells divide mitotically?
Replies: 1
Views: 4680

Why can't sperm cells divide mitotically?

Hi,,,
Can sperm cells or egg cells undergo mitotic division? I mean can a cell divide mitotically after meiosis? If not, why??? :roll:
Thank you
by Sue4
Tue Jul 30, 2013 8:49 pm
Forum: Molecular Biology
Topic: Bioinformatics and Wet experiments
Replies: 1
Views: 1876

Bioinformatics and Wet experiments

Hi, Can someone tell me what are the errors that can be occurred when using bioinformatics? For an example, it is always easy to use bioinformatics if we want to know the function of a particular protein. But can we be sure about the function that we obtained using bioinformatics without doing a lab...
by Sue4
Thu Apr 25, 2013 7:37 pm
Forum: Cell Biology
Topic: Carnoy's fixation problem
Replies: 0
Views: 3191

Carnoy's fixation problem

Hi everyone,
What should be the original concentration of ethanol or methanol used in the preparation of Carnoy's fixative solution? I know it is alcohol: glacial acetic acid( 3:1). But what is the original concentration of the alcohol?

Thank you very much :)
by Sue4
Sat Apr 20, 2013 8:10 am
Forum: Cell Biology
Topic: Plant chromosome staining
Replies: 0
Views: 2903

Plant chromosome staining

Hi everyone,
Can you please tell me what is the best stain to observe plant chromosomes and their aberrations (specially in onion cells) ? Feulgen stain or Aceto orcein? And are there any other suitable stains? Thank you very much
by Sue4
Wed Apr 17, 2013 3:44 pm
Forum: Molecular Biology
Topic: Staining Chromosomes
Replies: 1
Views: 3022

Staining Chromosomes

Hi,

Can you please tell me what is the best stain to observe plant chromosomes that allows to see chromosomal aberrations clearly. For an example onion chromosomes. Thank you
by Sue4
Mon Jan 28, 2013 9:03 pm
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 2494

"to be cloned" part is not needed. Sorry When making a gene construct (before inserting into an expression vector), do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the repor...
by Sue4
Mon Jan 28, 2013 8:23 pm
Forum: Molecular Biology
Topic: Orientation of genes
Replies: 2
Views: 2494

Orientation of genes

When making a gene construct to be cloned, do we have to place the desired gene, the reporter gene and the marker gene in any specific direction or orientation? I have seen in most cases the desired gene and the reporter gene have placed in one direction and the marker gene has placed in the opposit...
by Sue4
Fri Jan 25, 2013 11:43 am
Forum: Molecular Biology
Topic: Reporter gene without fusion
Replies: 1
Views: 2421

Reporter gene without fusion

I know that a reporter gene can be used to detect the foreign gene expression if it is fused with the foreign gene. But can we monitor the foreign gene expression if we place the reporter gene under a different promoter? If not, what is the use of placing a selectable marker and a reporter gene unde...
by Sue4
Thu Nov 29, 2012 6:18 pm
Forum: Molecular Biology
Topic: Have all human genes been identified?
Replies: 3
Views: 3161

Ohh..yes :O That's interesting. Thank you
by Sue4
Thu Nov 29, 2012 4:02 pm
Forum: Molecular Biology
Topic: Have all human genes been identified?
Replies: 3
Views: 3161

Have all human genes been identified?

Have all human genes been identified? If not, what was the reason? (Since it was one of the major goals of the human genome project )

Regards :)