Search found 15 matches

by mxnick262
Wed Apr 18, 2012 2:03 am
Forum: Molecular Biology
Topic: microRNA
Replies: 7
Views: 4605

Sorry I didn't respond sooner. One, citing wikipedia for an upper level biology course has to be the dumbest thing I've ever heard in my entire life. "You may want to use google scholar," like surely you don't actually receive a pay check for such sound advice. Two, you act as if your thre...
by mxnick262
Tue Apr 17, 2012 11:33 pm
Forum: Molecular Biology
Topic: Hard Question
Replies: 7
Views: 3319

FRET*
by mxnick262
Tue Apr 17, 2012 11:23 pm
Forum: Molecular Biology
Topic: Hard Question
Replies: 7
Views: 3319

BiFC. It's really a hypothetical question so even though this method has absolutely nothing to do with what we have learned this semester, it may still be right.

Still need one more method. GFP is to similar...
by mxnick262
Tue Apr 17, 2012 3:54 pm
Forum: Molecular Biology
Topic: Hard Question
Replies: 7
Views: 3319

Yea you are right. This question has me stumped.
by mxnick262
Tue Apr 17, 2012 3:47 pm
Forum: Molecular Biology
Topic: Transcription Factor Question
Replies: 7
Views: 4470

For how to determine the transcription factor binding sites in the whole genome, I'd use ChIP with ChIP sequencing. I'm more than 100% positive that's the way my professor expects us to do it since he just talked about Monday. As for the for the first question, I guess use a DNAse1 Foot-Printing Ass...
by mxnick262
Tue Apr 17, 2012 1:30 pm
Forum: Molecular Biology
Topic: Transcription Factor Question
Replies: 7
Views: 4470

Is chromatin immunoprecipitation wrong?
by mxnick262
Tue Apr 17, 2012 1:35 am
Forum: Molecular Biology
Topic: microRNA
Replies: 7
Views: 4605

Not understanding what I really want. I need an abstract PLUS the results of an experiment that illustrates a time when microRNA regulates mRNA. A simple link to a site with free journal access would have been a good reference. I don't want to pay for my example and I don't consider wikipedia a legi...
by mxnick262
Tue Apr 17, 2012 1:28 am
Forum: Molecular Biology
Topic: Hard Question
Replies: 7
Views: 3319

Please post something mildly useful. Methods that I have considered: protein-complex immunoprecipitation coupled with the use of some sort of molecular beacon? Guidance please.
by mxnick262
Tue Apr 17, 2012 1:23 am
Forum: Molecular Biology
Topic: Whole Genome Sequencing Technologies
Replies: 4
Views: 2784

Again you were of absolutely now help. I am not a graduate nor do I plan on getting a masters/PhD in this subject. I was looking for something along the lines of 454 sequencing, SOLiD sequencing, or illumina sequencing.
by mxnick262
Tue Apr 17, 2012 1:19 am
Forum: Molecular Biology
Topic: Transcription Factor Question
Replies: 7
Views: 4470

Re: Transcription Factor Question

ChIP coupled with ChIP-seq. Please do not mock me. I have obviously looked into it but, am looking for second opinions.
by mxnick262
Wed Apr 04, 2012 7:57 pm
Forum: Molecular Biology
Topic: Hard Question
Replies: 7
Views: 3319

Hard Question

You have performed yeast two hybrid assay to identify proteins that interact with superman and identified a gene coding for kryptonite that interacts with superman. What experiments you will perform to demonstrate that the superman interacts with kryptonite in-vivo (inside nucleus in the cell)? Give...
by mxnick262
Wed Apr 04, 2012 7:56 pm
Forum: Molecular Biology
Topic: microRNA
Replies: 7
Views: 4605

microRNA

What is microRNA?

Provide one example from literature how microRNA regulates mRNA.