Search found 7 matches

by cathbr
Thu Jul 18, 2013 3:56 pm
Forum: Molecular Biology
Topic: X chromosome inactivation
Replies: 1
Views: 3433

Hi Nicklowkc, X chromosome inactivation happens only in the presence of multiple X chromosomes. Male cells do contain only one X chromosome and do therefore not have X chromosome inactivation. X chromosome inactivation is happening through transcriptional repression of the X chromosome. The X chromo...
by cathbr
Fri Jul 05, 2013 6:53 pm
Forum: Cell Biology
Topic: adult stem cells, differentiated cells and proliferation
Replies: 2
Views: 5734

thanks! that was helpful!
by cathbr
Fri Jul 05, 2013 6:51 pm
Forum: Molecular Biology
Topic: HIS3-MX6 selective marker in S.cerevisiae
Replies: 0
Views: 2469

HIS3-MX6 selective marker in S.cerevisiae

Hi, I am facing some problems when I use HIS3-MX6 selection for budding yeast transformations. I was wondering if anybody had similar problems and would have a solution (like a specific way to transform yeasts with plasmids containing HIS3-MX). I try to transform an integrating PCR fragment that con...
by cathbr
Tue Jun 18, 2013 4:59 pm
Forum: Molecular Biology
Topic: PCR not consistent
Replies: 3
Views: 3699

What about the template that you are using? what is it? genomic DNA, plasmid,...? Are you sure that you are always using the same concentration and that the template quality is good and not degraded? and what kind of enzyme do you use?
by cathbr
Tue Jun 18, 2013 4:16 pm
Forum: Cell Biology
Topic: adult stem cells, differentiated cells and proliferation
Replies: 2
Views: 5734

adult stem cells, differentiated cells and proliferation

hi, I have two questions: 1) Are adult stem cells somatic cells? 2) Can differentiated cells proliferate in vivo? I mean once blood cells, nerve cells, muscle cells are differentiated do they actually proliferate if they are not cancerous? Or can only stem cells proliferate? I find the concept of ad...
by cathbr
Mon Mar 12, 2012 3:34 pm
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 8413

Hi, a lot of things could happen between the RNA and your PCR... If you give me more details I might be able to help For ex: the conditions that you are using for the PCR if you are using specific or random primers for the RT if you try to amplify the same products, or spliced varients,... with 4 di...
by cathbr
Mon Mar 12, 2012 1:46 pm
Forum: Molecular Biology
Topic: Telomerase activity in HEK293T cells
Replies: 0
Views: 1125

Telomerase activity in HEK293T cells

Does somebody know whether HEK293T cells are telomerase positive or ALT cell lines? I found in general difficult to know whether cell lines ar telomerase-positive or negative. Does somebody know papers in which there is like a summary of different cell lines and the way they maintain their telomeres...