Search found 6 matches

by Valya
Tue May 29, 2012 7:49 pm
Forum: Molecular Biology
Topic: Does urea disturb SDS interraction for SDS PAGE?
Replies: 1
Views: 1558

Does urea disturb SDS interraction for SDS PAGE?

If I add 8 M urea to the sample wouldn't SDS have concurense with it?
by Valya
Tue May 08, 2012 12:49 pm
Forum: Molecular Biology
Topic: an carbamylation of proteins take place without heating
Replies: 2
Views: 1782

an carbamylation of proteins take place without heating

Can urea degrade without heating? I mean can carbamylation of proteins take place without heating the probe?
by Valya
Thu Mar 29, 2012 6:59 pm
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 4445

Re:

JackBean wrote:Then try to cut the bands from gel and identify on MALDI or pepsin digest/LC-MS


Allready in progress :) Actually I have found a good question about tricine gel problem in Schägger protocol.. Will try it too.
by Valya
Thu Mar 29, 2012 6:55 am
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 4445

re: jonmoulton One person before me visulised it, but her probe didn't contain so much side substances.. My spectrum is so bad that I cant see there any peak. It's unpossible that the probe is empty, so that I have to decide what to eliminate.. I think about trying to cleave the pure probe without b...
by Valya
Wed Mar 28, 2012 8:42 pm
Forum: Molecular Biology
Topic: MALDI; fusion protein cleaving from a peptide
Replies: 6
Views: 4445

MALDI; fusion protein cleaving from a peptide

Hello, I have problem with cleaving a peptide (3,2 kDa) from a fusion protein.The construct contains protease site between fusion protein and the peptide of interest (actually I have 2 constructs: with enterokinase and with factor Xa site). Both proteases seem to work, but both show some nonspecific...
by Valya
Wed Feb 29, 2012 7:58 pm
Forum: Molecular Biology
Topic: Methionine for protein expression
Replies: 0
Views: 1157

Methionine for protein expression

If Methionine coding sequence is inserted in front of the sequence of protein in cloning vector, it facilitates an expression of this protein.
I need information about this technique. Can tou advise me some articles on this theme?