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by Rezaulbiox
Tue Feb 28, 2012 7:33 am
Forum: Molecular Biology
Topic: Designing Primer for over lapping PCR
Replies: 0
Views: 1375

Designing Primer for over lapping PCR

Hello everyone, I am trying to amplify a little bit long DNA of 6.72kb. I designed primer within 100 bp upstream and downstream of this DNA but failed to get the DNA by PCR. Some one suggested me to amplify the DNA by overlapping PCR. I do not know how to design the primer for overlapping PCR. I usu...