Zebrafish

Video introduction to microinjection: Rosen JN, Sweeney MF, Mably JD. Microinjection of zebrafish embryos to analyze gene function. J Vis Exp. 2009 Mar 9;(25). pii: 1115. doi: 10.3791/1115. http://www.jove.com/index/details.stp?ID=1115

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... DJ, Cooper HM, Hogan BM, Cody SH, Lieschke GJ, Heath JK. The Netrin receptor Neogenin is required for neural tube formation and somitogenesis in zebrafish. Dev Biol. 2004 May 1;269(1):302-15.

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... cause mutations or increase the probability that mutations will occur during meiosis, mitosis, or replication. My favorite mutation is the zebrafish mutant, one-eyed pinhead (oep). See slide 62 in this presentation: http://www.gene-tools.com/files/Philomath2008.ppt Regards, - Jon

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... of gene and there is always some risk. The two-nonoverlapping-oligo experiment is my favorite proof of specificity and is widely accepted by the zebrafish community. For this experiment, two Morpholino are made targeting the same RNA (for translation blockers) or the same pre-mRNA (for splice ...

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... be on the other side of the Morpholino target, in the intron, and cause a partial intron insertion. Draper BW, Morcos PA, Kimmel CB. Inhibition of zebrafish fgf8 pre-mRNA splicing with morpholino oligos: A quantifiable method for gene knockdown. Genesis. 2001 Jul;30(3):154-6. This one is available ...

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