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Vortex

vortex

Origin: L. Vortex, vertex, -icis, fr. Vortere, vertere, to turn. See Vertex.

1. A mass of fluid, especially of a liquid, having a whirling or circular motion tending to form a cavity or vacuum in the center of the circle, and to draw in towards the center bodies subject to its action; the form assumed by a fluid in such motion; a whirlpool; an eddy.

2. A supposed collection of particles of very subtile matter, endowed with a rapid rotary motion around an axis which was also the axis of a sun or a planet. Descartes attempted to account for the formation of the universe, and the movements of the bodies composing it, by a theory of vortices.

3. (Science: zoology) Any one of numerous species of small turbellaria belonging to Vortex and allied genera.

(Science: chemistry) Vortex atom, a hypothetical ring-shaped mass of elementary matter in continuous vortical motion. It is conveniently regarded in certain mathematical speculations as the typical form and structure of the chemical atom. Vortex wheel, a kind of turbine.


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Re: gel electrophoresis & assays help please

... Resuspend pellet in 1ml of fresh media and transfer to a clean eppendorf. [5] Add 50l of chloroform to each resuspended pellet, mix well (using vortex) and leave at room temperature for 5 min. [6] Pellet cell debris by centrifugation in microfuge for 10 min and full speed. Retain the supernatant ...

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by RachBio
Sun Feb 08, 2009 9:26 pm
 
Forum: Molecular Biology
Topic: gel electrophoresis & assays help please
Replies: 6
Views: 869

Protein degradation

I wouldn't leave it on the bench o.n. however you can vortex and put in 37deg for half an hour or so. It actually depends on what you'll do next and on the bugger (for western we put for 5 min in 95 and it's ok, right?). You could also try a different ...

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by GreenDog
Wed Jan 21, 2009 11:53 am
 
Forum: Molecular Biology
Topic: Protein degradation
Replies: 5
Views: 862

Re: Porblems making DIG probe - please help

... to the liberalized vector. The DIG mix contains everything and it’s the RNA mix. I know that polymerase buffers have a precipitant and I vortex and even heat a bit before use. Do you have any insight regarding incubation at 42 instead of 37? How is it best to purify the vector after restriction? ...

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by chaka8
Tue Jul 29, 2008 11:42 am
 
Forum: Molecular Biology
Topic: Porblems making DIG probe - please help
Replies: 5
Views: 980

plasma protein precipitation

... volume and incubate for 10 minutes and centrifuge at 1500 rpm; 10 minutes (rt) and after that, pour the supernatant --> do this 3 times. 2. Vortex the pellet and use Cell lysis solution (about 625 uL for 2,5 mL blood) & 2,5 uL RNase --> incubate for 30 minutes; 37C. 3. Do the protein ...

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by victor
Wed Jul 16, 2008 2:02 am
 
Forum: Molecular Biology
Topic: plasma protein precipitation
Replies: 1
Views: 782

Cleaning DNA from test tube

... to degrade the DNA, but rather to collect it. I have had this problem before. If it's a large construct like a BAC that you are purifying, *do not vortex* to resuspend. Try heating the solution at ~37C for 20 min. This should cause the DNA to resuspend in solution. You may still see a white pellet ...

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by snowcapk
Tue May 13, 2008 3:56 am
 
Forum: Molecular Biology
Topic: Cleaning DNA from test tube
Replies: 4
Views: 775
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