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Vectors

vector

1. (Science: mathematics) A term to describe something that has both direction and magnitude.

2. (Science: molecular biology) Commonly term for a plasmid that can be used to transfer dNA sequences from one organism to another. Different vectors may have properties particularly appropriate to give protein expression in the recipient or for cloning or may have different selectable markers.

recombinant DNA systems especially suited for production of large quantities of specific proteins in bacterial, yeast, insect, or mammalian cell systems.

See: transfection.


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WHO scum - politics over lives

... areas is in dire need of change. There are so many factors that are contributing to the overall squalor. Programs such as WHO eliminating vectors and other organizations handing out food are noble, but they are only a bandaid on a severely infected wound. They are short-term aid while, ...

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by AstusAleator
Wed Jun 10, 2009 6:27 pm
 
Forum: Ecology
Topic: WHO scum - politics over lives
Replies: 15
Views: 689

Cure for AIDS

Do you know if lentivirus based vectors (allowing stable transduction of cells) insert to host DNA at specific sequences or randomly? I know it is a replication independent process.

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by kk
Fri Apr 17, 2009 1:26 pm
 
Forum: Microbiology
Topic: Cure for AIDS
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Re: Important unknown nanotech within humans

... livestock and plants in the country, and keeping diseases from crossing into Australia. He was an expert in the propagation of diseases by insect vectors, among other things. —Circumstance of Death: He died along with 15 other people when the commuter plane he was traveling in went down in Queensland, ...

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by vincio
Mon Feb 16, 2009 2:36 am
 
Forum: Molecular Biology
Topic: Important unknown nanotech within humans
Replies: 28
Views: 6293

Why is bacteria good?

... Just bear in mind that also eukaryotic cells can be used in similar manner; yeasts are often used, as are insect cells (with e.g. baculovirus vectors) and even human cells. But this is where the benefits of bacteria can be seen: they are much more simple and cheap to grow than cells from higher ...

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by biohazard
Fri Feb 13, 2009 9:39 pm
 
Forum: Microbiology
Topic: Why is bacteria good?
Replies: 12
Views: 1742

Re: Amount of DNA for eficient transformation

not much at all is necessary. for example, commercially available vectors for transformation efficiency control are transformed at the pg level. however, if u are using ligation products, i used something like 50ng.

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by pcrboy
Thu Jan 29, 2009 12:10 pm
 
Forum: Molecular Biology
Topic: Amount of DNA for eficient transformation
Replies: 4
Views: 533
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