Terminator

(starting concentration) * (starting volume) = (ending concentration) * (ending volume) or restated more concisely, C1V1=C2V2 You know your desired ending concentration and volume. You need to find out how much of your starting material to use. So, V1=C2V2/C1

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Hi : I need to sequence a DNA. My primer conc. is 10 micromol and I need to convert it to 3.2 picomol to be able to set uo my sequencing reaction? how can I dilute my primer (10 micromol) to reach this small value of conc (3.2 picomol) ???? Anyone can explain to me in detail please?

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... manufacture of trp. In this case (low trp levels - want to produce trp) attenuation will not occur (because the ribosome stalls at attenuator, antiterminator forms, transcription can proceed to trp genes) and so expression will proceed CONSTITUTIVELY until a certain point where there is enough ...

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... to see what is going on....all in all, looking back over the entire threads, there were not that many attacks, or problems...only since the Terminator showed up. Please, give it some thought - what I am saying should be obvious even to novices in "military tactics".... Let Barz ...

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I would like to use Epicenters Terminator 5'-phosphate-dependent exonuclease to digest human rRNA, but don't want to destroy any viral RNA present in the sample. The enzyme only digests RNA with a 5'-monophosphate, not those with a cap, ...

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