Dictionary » S » Substrate

Substrate

Definition

noun, plural: substrates

(geology)

A substratum or an underlying stratum.

(chemistry)

The substance acted upon by an enzyme.

(biology)

The earthy material in which an organism lives, or the surface or medium on which an organism grows or is attached.

(marine biology)

The material (e.g. dirt, rocks, sand, gravel) in the bottom of a marine habitat, or one that forms the bed of a stream (or of an aquarium); the source of food for some microorganisms.


Supplement

Word origin: L. Stratum = layer.

Related terms: substrate inhibition, substrate-level phosphorylation, substrate specificity, suicide substrate, enzyme-substrate complex.

Synonym: substratum.


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Enzymes and activation energy.

Enzymes attach to a substrate and weaken intermolecular forces within the substrate and thus weaken their bonds so the reaction takes place much quicker. As you see otherwise it would take a greater amount of time for a reaction to ...

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by prokaryotics
Sat Jul 11, 2009 9:09 am
 
Forum: Molecular Biology
Topic: Enzymes and activation energy.
Replies: 4
Views: 380

product-vs-time experiment of amylase

if i were to guess (and this is a guess) i would say you have too much substrate in your reaction. Because your reaction speed is limited by the amount of enzyme, not the amount of substrate, adding more substrate doesn't make a difference (essentially I'm ...

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by MrMistery
Sat Jul 11, 2009 4:50 am
 
Forum: Molecular Biology
Topic: product-vs-time experiment of amylase
Replies: 3
Views: 173

product-vs-time experiment of amylase

Any chance you made a mistake with the substrate (or possibly the enzyme) concentration and you are saturating the enzyme at all substrate levels?

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by blcr11
Fri Jul 10, 2009 10:51 pm
 
Forum: Molecular Biology
Topic: product-vs-time experiment of amylase
Replies: 3
Views: 173

product-vs-time experiment of amylase

... I was working with new enzymes (well new to me) today in a biochemistry lab and had to determine the velocity of the reaction for three different substrate conditions. Generally, when I plot the graphs, there are 3 different lines, corresponding to the different concentrations. However today when ...

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by NewtoBiology
Fri Jul 10, 2009 9:24 pm
 
Forum: Molecular Biology
Topic: product-vs-time experiment of amylase
Replies: 3
Views: 173

Re: Lab of Vmax and KM

That's it. The velocity approaches Vmax asymptotically as the substrate concentration increases. However, changing other conditions can change the Michaelis–Menten plot -- for instance, a different pH or a different temperature can lead to a different relationship ...

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by jonmoulton
Thu Jul 09, 2009 4:00 pm
 
Forum: Molecular Biology
Topic: Lab of Vmax and KM
Replies: 1
Views: 185
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