Staining

I am a bit surprised here, you say that post satining is too time consuming but you are ready to slow down your gels. It takes 10 minutes for staining, and about as much if you want to destain, which should not even be a problem with gelred. So why not post stain? If this is really not an option, ...

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... MrMistery said, include many of the standard methods of cellular and molecular biology, including PCR, different blottings, monocolonal antibody staining/blocking, knock-out and/or transgenic organisms (cells, bacteria, plants, animals, fungi), gene chips, microscopy (electron, confocal, standard ...

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... on the objective you're using? Third, you say you found an organism living in a human. Have you taken any steps to identify it's domain? Any staining at all? (ie, gram +/- or nuclear staining, etc..) It's near impossible to identify an organism with the information you provided.

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I am performing SDS-PAGE with mitochondrial fractions. after transfering the protein in nitrocellulose membrane when i'm staining the same with Ponceau's solution, i am getting distinct bands at higher molecular weight region but not getting anything below 35 kD region. i'm approximately ...

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... work with imageJ software with colour deconvolution (detection of DAB stain) and i need for validity this article: Quantification of histochemical staining by color deconvolution. Anal Quant Cytol Histol 23: 291-299, 2001 if someone has this article and has been kind enough to give me send me to ...

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