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Shearing

Shearing

1. The act or operation of clipping with shears or a shearing machine, as the wool from sheep, or the nap from cloth.

2. The product of the act or operation of clipping with shears or a shearing machine; as, the whole shearing of a flock; the shearings from cloth.

3. Same as Shearling.

4. The act or operation of reaping.

5. The act or operation of dividing with shears; as, the shearing of metal plates.

6. The process of preparing shear steel; tilting.

7. (Science: chemical) The process of making a vertical side cutting in working into a face of coal. Shearing machine. A machine with blades, or rotary disks, for dividing plates or bars of metal. A machine for shearing cloth.

Can someone help me define as to avoid *skin shearing* in elderly patient


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Lookin for a genomic DNA extraction kit for bacteria

... samples and they consistantly extract all kind of DNA correctly. But it depends what application you have for your DNA. Many kit do creat a lot of shearing in your DNA, and that is not always a Good Thing.

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by canalon
Fri Mar 07, 2008 6:15 pm
 
Forum: Molecular Biology
Topic: Lookin for a genomic DNA extraction kit for bacteria
Replies: 7
Views: 469

Smeary restriction digest of genomic DNA

... complete with low molecular weight bands indicative of histonal DNA. I'm pretty sure it's more due to nuclease contamination than mechanical shearing, though there may some shear as well. The original preps were also run on the gel, and look fine for all samples. Because the same reagents ...

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by icequeen11
Tue May 22, 2007 7:43 pm
 
Forum: Molecular Biology
Topic: Smeary restriction digest of genomic DNA
Replies: 1
Views: 495

Question about DNA conformation

Shearing does not destroy the helix... So you'd have dsDNA fragments of <2000bp. How long did you allow the DNA to disolve? I've used ssDNA and it takes a while for it to completely dissolve (I left it overnight at 4C and ...

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by LilKim
Mon Jan 22, 2007 7:29 pm
 
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 1053

Question about DNA conformation

... water. Once dissolved, the DNA solution is sheared to an average size of <2000bp and the concentration is adjusted to 10mg/ml..." Does the shearing process destroy the helixity of the DNA? Upon diluting the DNA, some undissolved material was observed (the solution was not clear due to this ...

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by ahkian82
Mon Jan 22, 2007 6:40 am
 
Forum: Molecular Biology
Topic: Question about DNA conformation
Replies: 4
Views: 1053

The Fiber Disease

... be read several times to correct for errors. This article has details on the process used by this sequencing instrument. The 454 approach involves shearing the starting material DNA using a nebulizer. Rothberg explains: “[We] nebulize the DNA into little fragments, shake it in oil and water, so ...

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by London
Tue Dec 12, 2006 11:09 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 748674
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