Screening

Hi there, what exactly genome wide screening? I guess it is when you look at genes for mutations etc? How is it done? Thanks in advance, b_06er

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Try silk screening...and transfer to a media with pure horse blood.

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OK, I found a method that works: Hydroxylamine Mutagenesis. I got sooo many mutants, now it's time for screening... I tried one protocol that asked for incubation at a high temp for a short amount of time. This didn't make sense to me since hydroxylamine is denatured at high ...

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... I HAVE A QUESTION ABOUT THE TOPIC. IF SOME ONE CAN HELP ME, PLEASE, I NEED IT. I AM DESIGNING PCR OLIGOS, AND IN THE FUTURE WE COULD USE THEM TO SCREENING A DNA LIBRARY. IS TOTALLY IMPORTANT THAT THE PRIMERS ARE IN READING FRAME? IF THE ANSWER IS YES, PLEASE TELL ME HOW TO DO IT. THANKS. I AM ...

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... DO) how parallel cell density results could be achieved? Let say if standard deviation of cell density is about 15% would this be acceptable when screening different environmental conditions. Of source there is some error in manual calculation of cells that can be included in 15%.

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