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Samples

sample size

The number of units (persons, animals, patients, specified circumstances, etc.) in a population to be studied. The sample size should be big enough to have a high likelihood of detecting a true difference between two groups.


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transform xy-data to xyz-data

... good reason to do that, and considering what we know about you dataset (not much) I guess that the only z dimension that you have is the number of samples for each particular (X,Y) coordinates, but that will make sense only if there are more than one of each pair.

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by canalon
Thu Sep 03, 2009 3:22 pm
 
Forum: Bioinformatics
Topic: transform xy-data to xyz-data
Replies: 1
Views: 18

Microarray analysis - SAM

... most grateful if someone could please tell me how to calculate the number of permutations in SAM analysis. I know that it depends on the number of samples being analysed, and that it requires the factorial of this number - but have not been able to find the correct formula! Thank you!

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by DrCl
Tue Sep 01, 2009 12:24 pm
 
Forum: Molecular Biology
Topic: Microarray analysis - SAM
Replies: 0
Views: 33

separation problem with GelRed

... in our lab. (We always prestain gels - poststaining is no option because its too inconvenient and timeconsuming - using 1xTAE buffer). Our DNA samples and the ladders aren't separated as sharply as it was when we used ethidium bromide. I already have some ideas what to try to improve the separation ...

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by apolll
Sun Aug 23, 2009 8:23 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 1
Views: 70

PCR & Agarose gel electrophoresis

Hi there, I have been trying to determine the genotype of some cell samples using PCR and agaorse gel electrophoresis. On one of the samples, I was supposed to find two bands representing a heterozygote for a particular mutated receptor but I could only see ...

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by Juanita1
Thu Aug 20, 2009 8:21 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 127

Cell counting question

... and concentrated sample. All of these came from the same initial fixed stock. We also did a differential count for dividing cells using the same samples My weird results showed up between my control and my concentrated samples. Since my cells were fixed, I expected to have the same ratios between ...

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by jb0378
Thu Aug 20, 2009 5:11 pm
 
Forum: Cell Biology
Topic: Cell counting question
Replies: 7
Views: 137
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