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Room temperature


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Re: [molecular bio]calculation

... of cholesterol (mol. wt. 386.7) Acetic acid (CH3COOH) is a liquid at room temperature. When making acetate buffers (as in the question above) you must add specific molar ...

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by scholesmu
Sun Aug 16, 2009 11:49 am
 
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 124

Re: Ligation and expression trouble

... pretty much lost." My question is: Ligase has 100% activity at room temperature then how can it go bad when left at RT? 2)Since I used two different restriction ...

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by solution
Wed Jul 08, 2009 9:16 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 398

Anybody knows how stable is ssDNA??

... at my bench. Anybody has and information about how stable is ssDN at room temperature? especially in that buffer? Please help, my background in biochemistry is not that ...

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by primary
Tue Jun 09, 2009 11:08 pm
 
Forum: Molecular Biology
Topic: Anybody knows how stable is ssDNA??
Replies: 2
Views: 173

problems with MTS test

... (1), I tested the effect of glycerol on the viability of the tissues at room temperature (2), and added a positive control (only tissue in culture medium) (3), and a negative ...

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by rsegreto
Wed Jun 03, 2009 10:51 am
 
Forum: Cell Biology
Topic: problems with MTS test
Replies: 0
Views: 197

Some problems of DNA recovery from native PAGE gel

... (100ug gel ~ 200ul TE buffer)., rotating the mixture for overnight at room temperature (~24C); 6. centrifuge the mixture at 12000g for 15 min; 7. take out the supernatant, ...

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by wusilinqi
Thu May 14, 2009 5:20 am
 
Forum: Genetics
Topic: Some problems of DNA recovery from native PAGE gel
Replies: 1
Views: 550
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