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Reverse transcription

Definition

noun

The process of making a double stranded DNA molecule from a single stranded RNA template through the enzyme, reverse transcriptase.


Supplement

It is called reverse transcription because it is a process in opposite or reverse of transcription.

See also: reverse transcriptase.


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Central Dogma of Molecular Biology

... of DNA, with James Watson, in 1953. The conversion of DNA to mRNA is transcription, and the conversion of mRNA to the protein is translation. ... back to DNA by retroviruses (RNA viruses) such as HIV with the use of reverse transcriptase enzyme. Now, I ask, How does this process of DNA, mRNA, ...

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by JosePR
Sat Apr 25, 2009 8:53 pm
 
Forum: Molecular Biology
Topic: Central Dogma of Molecular Biology
Replies: 3
Views: 1060

Re:

... by replication of two existing strands when they are unwound, NOT transcription. ;) Which is referred to as replication and is the normal process ... Actually it is possible to produce DNA from transcription, via reverse transcription. This happens when certain viruses such as HIV invades ...

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by Sepals
Wed Mar 11, 2009 2:11 pm
 
Forum: Molecular Biology
Topic: DNA and RNA
Replies: 6
Views: 1207

RAPD and viruses

... be sure) -> checkpoint: it works, we have RNA on agarose gel -> Reverse Transcription (Fermentas RevertAid), one cycle with random hexamers -> checkpoint: how? ...

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by AdamAdam
Sat Aug 16, 2008 11:40 am
 
Forum: Molecular Biology
Topic: RAPD and viruses
Replies: 0
Views: 961

Questions about single pig oocyte RT-PCR.

... to remove cumulus cells. The amplification program for the reverse transcription step was as follows: 70°C for 10 min, ice 2min ,MMLV ,42°C for 50min, 95°C for ...

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by dragonwang
Mon Jun 23, 2008 3:48 am
 
Forum: Molecular Biology
Topic: Questions about single pig oocyte RT-PCR.
Replies: 1
Views: 598

virology

... and replicate via a DNA intermediate. Retroviruses rely on the enzyme reverse transcriptase to perform the reverse transcription of its genome from RNA into DNA, which can then be integrated into the host's ...

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by rabia
Tue May 06, 2008 7:32 pm
 
Forum: Microbiology
Topic: virology
Replies: 3
Views: 1402
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