Dictionary » R » Restriction

Restriction

restriction

1. The process with which foreign dNA that has been introduced into a prokaryotic cell becomes ineffective.

2. A limitation.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


Re: Ligation and expression trouble

... eventually break down. T4 ligase works just fine at RT, but you can't reuse it now, can you? In fact, that is the main paradigm with enzymes. take restriction enzymes: most of them are active at 37C, but they need to be stored at -20C, and even when you take them out of the freezer you should keep ...

See entire post
by MrMistery
Thu Jul 09, 2009 12:41 am
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Re: Ligation and expression trouble

... much lost." My question is: Ligase has 100% activity at room temperature then how can it go bad when left at RT? 2)Since I used two different restriction enzymes producing overhangs of different bases how can the vector religate, in other words how would the CIP help, wouldnt using CIP make ...

See entire post
by solution
Wed Jul 08, 2009 9:16 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Re: Ligation and expression trouble

... We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction ...

See entire post
by MrMistery
Wed Jul 08, 2009 3:18 am
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Ligation and expression trouble

... We ordered pOTB7 vector with gene A from a company and constructed deletion constructs of the gene A from above vector. We designed primers with restriction sites for 4 deletion constructs, PCR amplified and gel purified the fragments using Qiagen kit. Using the same enzymes (Kpn1 and XbaI) restriction ...

See entire post
by solution
Mon Jul 06, 2009 7:36 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Re: Cancer and Cell cycle - cyclins/cdk/mutated genes?

... are all thru out the mammalian cell cycle: Mid-G1 cyclin CDKs - Cyclin D-CDK4, Cyclin D-CDK6 (complexes form in mid G1 phase and peak at the restriction point, then taper off at S phase) Late-G1 cyclin CDK - Cyclin E-CDK2 (complexes starts right after the restriction point, peaks at the beginning ...

See entire post
by kolean
Mon Jul 06, 2009 7:08 pm
 
Forum: General Discussion
Topic: Cancer and Cell cycle - cyclins/cdk/mutated genes?
Replies: 1
Views: 169
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 1,141 times. 
What links here | Related changes | Permanent link