Relaxed plasmid

... diversion. All definitions are from Biology Onlines Dictionary Plasmid : (Science: molecular biology) A small, independently replicating, ... replication. stringent plasmids occur at low copy number in cells, relaxed plasmids at high copy number, ca 10 to 30. plasmids can become incorporated ...

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... and uncut vector with one site only, I can see that the supercoil and relaxed form are now all liniar at the right size , but there is a weak band ... this band is also in the uncut vector. it seems that in this form of plasmid the digestion is inhibited. does anyone has this problem also? do ...

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